Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant)

Article Abstract:

Pseudomonas aeruginosa ATCC 9027 produces a rhamnolipid which can act as a biosurfactant. The ability of this biosurfactant to increase the aqueous dispersion of slightly soluble molecules was quantitated through the effects of rhamnolipid concentration, pH and shaking speed. The results showed that 300 mg per liter of rhamnolipid could markedly increase dispersion of octadecane solutions, which was also dependent on pH and shaking speed. The same concentration of rhamnolipid could increase the biodegradation rate of octadecane by 15% compared to the control. These results demonstrate the usefulness of rhamnolipids in remediating hydrocarbon-contaminated water.

author: Zhang, Yimin, Miller, Raina M.
Usage, Lipids

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Influence of rhamnolipid biosurfactant on the transport of bacteria through a sandy soil

Article Abstract:

A modified advection-dispersion transport model was utilized to determine the influence of anionic monorhamnolipid biosurfactant on the irreversible adsorption of various Pseudomonas (P.) aeruginosa strains. Analysis of the bacterial cell breakthrough curves of of P. aeruginosa ATCC 9027, ATCC 27853 and ATCC 15442 in glass chromatography columns indicated the role of rhamnolipid in stimulating cell adsorption. Rhamnolipid also enhanced the negative surface charge density of the glass chromatography column without altering its surface charge density.

author: Brusseau, Mark L., Miller, Raina M., Bai, Guiyun
Models, Bacteria, Pseudomonas aeruginosa, Cell migration, Chromatography, Bacterial motility, Chromatographic analysis

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Rhamnolipid (biosurfactant) effects on cell aggregation and biodegradation of residual hexadecane under saturated flow conditions

Article Abstract:

Microbiological research shows that rhamnolipid biosurfactant causes hexadecane to mobilize in sand and could be useful in pollution clean-up operations. Biodegradation occurs at lower biosurfactant concentrations. The effectiveness of a biosurfactant is impacted by the presence and nature of microorganisms. Further research is required to establish how the biosurfactant reacts with microogansims.

author: Zhang, Yimin, Miller, Raina M., Herman, David C.
Pollution, Hydrocarbons, Surface active agents

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subjects list: Research, Biodegradation
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