Article Abstract:
Temperature-resistant suppresor mutants derived from Escherichia coli W3110 were constructed to increase heterologous protein production. Two mutants, MON102 and MON105, were found to be defective in the rpoH gene (rpoH358) involving a cytosine to thymine transition at nucleotide 802. A 1.3- to 1.7-fold increase in heterologous protein production was detected in the mutant strains as compared to the wild-type. The enhanced levels of proteins were associated with increased synthesis of corresponding mRNA and translated proteins, indicating protein accumulation was not due to decreased proteolysis.
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Article Abstract:
Two rapid, plate-assay-based screening techniques were developed and applied for identifying manganese(IV) (Mn(IV)) reduction-deficient (Mnr) mutants of Shewanella putrefaciens 200. The assay was developed because of the inability of S. putrefaciens 200 to form anaerobic colonies on solid medium supplemented with Mn(IV). Two specific respiratory Mnr mutants of S. putrefaciens were identified using the assays. These mutants were unable to respire anaerobically on Mn(IV). However, they were able to respire anaerobically using other compounds as sole terminal electron acceptor.
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Article Abstract:
Mutants of Clostridium butyricum E5, which resist allyl alcohol (AA), produce less butyrate than acetate and consume less time, whereas the wild type produce equal quantities of butyrate and acetate and take more time. Both strains show similar properties while converting glycerol. AA-resistant mutants exhibit slightly decreased levels of glycerol dehydrogenase compared to the wild type. The acetate-butyrate formation is the major activity through which the mutants regulate the internal redox balance.
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