Article Abstract:
A bacterial catalyst for bioconversion of the aromatic hydrocarbon toluene and several alkyl and chloro- and nitro-substituted derivatives into the corresponding benzoates was developed by fully reassembling the upper TOL operon of plasmid pWWO of Pseudomonas putida as a single gene cassette along with its cognate regulatory gene, xylR. To match the corresponding DNA segment to the chromosome of a P. putida strain, a genetic method was used that facilitates the elimination of all recombinant tags carried over from earlier cloning steps and that leaves the natural strain bearing only the relevant DNA segment. The results are discussed.
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Article Abstract:
Organophosphoric compounds (OPCs) belong to a class of pesticides in use around the world. They account for a large number of poisonings in humans and pose hazards for the environment. Thus, microbial degradation of the compounds are of great interest for researchers. In an effort to better understand the metabolic process behind the microbial utilization of OPCs. spontaneous organophosphate utilization mutants of the Escherichia coli K-12 strain was investigated. Two genes responsible for the mutation were then identified using cloning experiments.
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Article Abstract:
The regulation of naphthalene and 1-naphthol degradation occurs independently and through separate pathways in Rhodococcus sp. strain NCIMB 12038. A monooxygenase enzyme may be involved in the degradation of indene and the biotransformation of indole to indigo. Growth on salicylate represses the metabolism of naphthalene and 1-naphthol. The degradation of naphthalene involves salicylate as a central metabolite. It is oxidized by a naphthalene dioxygenase enzyme to cis-(1R,2S)-1,2-dihydroxy-1,2-dihydronaphthalene.
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