Biotransformation of various substituted aromatic compounds to chiral dihydrodihydroxy derivatives

Article Abstract:

The chlorobenzene dioxygenase from Pseudomonas sp. strain P51 converts substituted aromatic compounds to the S configuration at the carbon atom. The enantiomeric excess was higher than the same reaction catalyzed by toluene dioxygenases. This is of interest to the biotechnology industry because cis-dihydrodiols are building blocks for molecules with multiple chiral centers.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Enantioselective transformation of alpha-hexachlorocyclohexane by the dehydrochlorinases LinA1 and LinA2 from the soil bacterium Sphingomonas paucimobilis B90A

Article Abstract:

The enantioselective transformation of alpha-hexachlorocyclohexane (HCH) by LinA1 and LinA2 variants expressed as S-glutathione transferase (GST) fusion proteins in Escherichia coli BL21 is reported. Enantioselective degradation might be one of the key processes that determine enantiomer composition, especially when strains that contain only one of the linA genes, such as Sphingmonas paucimobilis UT26, prevail.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Enantioselective metabolism of chiral 3-phenylbutyric acid, an intermediate of linear alklybenzene degradation, by Rhodococcus rhodochrous PB1

Article Abstract:

The degradation of chiral 3-phenylbutyric acid by the gram-positive bacterium Rhodococcus rhodochorus PB1 was investigated. Only one enantiomer was used as a growth substrate. Data indicated that active cells were able to cometabolize the non-growth enantiomer to a dihydroxylated intermediate. Data also showed the importance of treating enantiomers separately in studies of chiral compound degradation.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: