Article Abstract:
A study was conducted on the Rhodococcus sp. strain IGTS8 to determine its dibenzothiophene (DBT) desulphurization pathway. Sulfur-containing products were chemically synthesized and incubated with whole cells of strain IGTS8 to identify the accurate path from DBT to 2-hydroybiphenyl. Results have shown that DBT-desulphurization (dsz) operon gene product DszC is responsible for the S-oxidation, DszA for the conversion of DBTO2 to 2-(2'-hydrophenyl)benzen sulphinate (HBPSi-) and DszB for the desulphination of HBPSi-.
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Article Abstract:
A kinetic approach by electrolytic respirometry with the Sapromat apparatus was used to characterize uptake mechanisms of polycylic aromatic hydrocarbons. The approach utilized a biphasic medium, determined the physicochemical phase transfer rates and biodegration rates, and interpreted the kinetic data in terms of mathematical models of uptake mechanisms. Degradation of pyrene by a Rhodococcus sp. showed the existence of a different substrate mechanism.
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Article Abstract:
Research was conducted to examine the intracellular survival and saprophytic growth of isolates from the Burkholderia cepacia complex in free-living amoebae. It was observed that clinical and environmental strains of the B. cepacia complex can survive within different isolates of the genus Acanthamoeba. Results indicate that free-living amoebae may be a reservoir for the acquisition, and perhaps the transmission of B. cepacia.
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