Elimination of the yeast RAD6 ubiquitin conjugase enhances base-pair transitions and G . C to T. A transversions as well as transposition of the Ty element: implications for the control of spontaneous mutation

Article Abstract:

The RAD6 gene that encodes the enzyme for protein ubiquitination causing spontaneous mutagenesis was investigated using DNA sequencing. Spontaneous mutations that arose in yeast suppressor tRNA gene SUP4-o were characterized. Results from the mutational spectrum were compared to findings from mutational spectrum of isogenic wild-type parent. The mutation resulted in the absence of the enzyme and consequently increased the rate of base pair transitions and G. C to T. A transversions. Ty transitions were also observed to be enhanced in the yeast.

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A ubiquitin-conjugating enzyme, RAD6, affects the distribution of Ty1 retrotransposon integration positions

Article Abstract:

The effect of mutations in rad6, the gene encoding the ubiquitin-conjugating enzyme RAD6, on target site preferences of the yeast transposons Ty1 and Ty2 was assessed. The 53 independent Ty1 and Ty2 insertions isolated in an isogenic rad6 deletion strain did not show the conventional gradient of insertion frequency, rather, the Ty elements were inserted randomly throughout the CAN1 gene. Thus, the ubiquitin-conjugating enzyme, RAD6, alters the integration site preferences of Ty1 retrotransposons.

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Specificities of the Saccharomyces cerevisiae rad6, rad18, and rad52 mutators exhibit different degrees of dependence on the REV3 gene product, a putative nonessential DNA polymerase

Article Abstract:

REV3-dependent DNA damage processing affected specification for mutator forms of yeast rad6, rad18 and rad52. Specification differences accounted for the functional value of REV3 in spontaneous mutagenesis. Thus, mutator behavior in the repair of DNA damage in Saccharomyces cerevisiae was influenced by REV3 dependence. The REV3 gene product was also associated with the occurrence of DNA lesions.

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