Efficacy of chlorine and heat treatment in killing Salmonella stanley inoculated onto alfalfa seeds and growth and survival of the pathogen during sprouting and storage

Article Abstract:

Treatment of alfalfa seeds containing Salmonella stanley with a 2,000- to 4,000-micro-g/ml chlorine (Cl) solution decreases the bacterial content without affecting seed germination. However, the bacterial content may increase when the seeds sprout. Dipping the seeds in a solution containing 290 micro-g of Cl per ml significantly decreases the bacterial content while treatment with a solution of 1,010 micro-g of Cl per ml has no further effect. Heat treatment at 57-60 degrees C for 5 mins kills bacteria without affecting germination while treatment for 10 mins inhibits germination.

author: Beuchat, Larry R., Mahon, Barbara E., Jaquette, Cynthia B.
Usage, Salmonella, Control, Microorganisms, Disinfectants, Alfalfa, Chlorine, Chlorine and derivatives as disinfectants, Temperature effects, Grain seeds

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Effects of nisin and temperature on survival, growth, and enterotoxin production characteristics of psychotropic Bacillus cereus in beef gravy

Article Abstract:

The growth of four strains of psychotropic Bacillus cereus strains were cultured in a brain heart infusion (BHI) broth and beef gravy with nisin supplementation. The repair of cytoplasmic membrane dysfunction in heat injured Bacillus cereus cells was inhibited by nisin which acted as a voltage-dependent polarizer that prevented the restoration of membrane integrity. The growth and enterotoxin synthesis by Bacillus cereus in BHI was also attenuated by nisin compared to microbial cultures in beef gravy.

author: Beuchat, Larry R., Jaquette, Cynthia B., Clavero, M. Rocelle S.
Research, Physiological aspects, Bacterial growth, Enterotoxins, Cell culture, Tissue culture, Bacillus cereus

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Evaluation of chemicals for restricting colony spreading by a xerophilic mold, Eurotium amstelodami, on dichloran-18% glycerol agar

Article Abstract:

Twenty chemicals were tested to determine their effectivity in restricting colony speading of four strains of Eurotium amstelodami on dichloran-18% glycerol agar. Of the chemicals, the most effective were Triton X-100, Triton X-301, Tergitol NP-7 and Tergitol 15-S-3 at 200 micrograms per milliliter, and 1,000 micrograms of sodium deoxycholate, 1 microgram of iprodione, 0.1 microgram of propiconazole and 0.01 microgram of Maxim per milliliter.

author: Beuchat, Larry R., Tapia, Maria Soledad de Daza
Evaluation, Antifungal agents

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