Effects of long-term storage on plasmid stability in Bacillus anthracis

Article Abstract:

The effects of long-term storage on the stability of both virulence plasmids (pXO1 and pXO2) are assessed by studying the plasmid profiles of a large collection of Bacillus anthracis isolates that were stored at the Centers for Disease Control and Prevention from the 1950s through 1980s. There was no significant difference in the proportion of strains containing only pXO1 or strains containing only pXO2, but there was interdependence between the two plasmids.

author: Johnson, Scott, Popovic, Tanja, Marston, Chung K., Hoffmaster, Alex R., Wilson, Kathy E., Bragg, Sandra L., Plikaytis, Brain, Brachman, Philip, Kaufmann, Arnold F.
Methods, Polymerase chain reaction

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Development and implementation of a single-chain Fv antibody for specific detection of Bacillus anthracis spores

Article Abstract:

A single-chain Fv (scFv) antibody is developed and implemented for specific detection of Bacillus anthracis spores. The potential utility of scFv antibodies in detection platforms is shown by the application of the soluble purified scFv antibody in enzyme-linked immunosorbent assays, immunofluorescence assays, and flow cytometry (FCM).

author: Zahavy, E., Fisher, M., Mechaly, A.
Technology application, Flow cytometry, Immunofluorescence, Fluorescent antibody technique, Viral antibodies, Antibodies

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Plasmid-mediated high-level gentamicin resistance among enteric, bacteria isolated from pet turtles in louisiana

Article Abstract:

A study was conducted to identify the gene(s), which contributes to the high-level gentamicin resistance phenotype observed in bacteria from environmental samples with turtle farming activity. The results indicated a dissemination of the gentamicin resistance genes through mobile molecular elements.

author: Diaz, Maria Alejandra, Cooper, Richard Kent, Clocckaert, Axel, Siebeling, Ronald John
Physiological aspects, Gentamicin, Gentamicins, Turtles as pets, Pet turtles

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subjects list: Research, Genetic aspects, Plasmids, Bacillus anthracis
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