Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria

Article Abstract:

The aim of the study was to investigate the effects of growth medium, inoculum size, colony density, and incubation time on the appearance of colonies of members of these poorly studied groups of soil bacteria on plates of solid growth media inoculated with soil. The successful isolation of members of groups of bacteria that are widely distributed and common in soils worldwide seems to be a result of using appropriate media and extended incubation times.

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Detection and cultivation of soil Verrucomicrobia

Article Abstract:

A simple approach that has generated a collection of new strains of the phylum Verrucomicrobia is reported. The analysis has shown that colony development by members of the class 'Spartobacteria' (subdivision of the phylum Verrucomicrobia) is inhibited by other culturable bacteria.

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Laboratory cultivation of widespread and previously uncultured soil bacteria

Article Abstract:

Collections of 350 isolates from soil were cultured by using simple solid media in petri dishes. The findings state that many of the bacteria are readily culturable. The isolation also allows investigation of the properties of these novel bacteria.

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