Effectiveness of disinfectants in killing Enterobacter sakazakii in suspension, dried on the surface of stainless steel, and in a biofilm

Article Abstract:

A study was conducted to determine the effectiveness of 13 disinfectants used in hospitals, day-care centers, and food services kitchens in killing Enterobacter sakazakii in suspension, dried on the surface of stainless steel, and in biofilm. The findings show that disinfectants routinely used in hospital, day-care, and food service kitchen settings are ineffective in killing some cells of Enterobacter sakazakil embedded in organic matrices.

author: Beuchat, Larry R., Kim, Hoikyung, Ryu, Jee-Hoon
Polishes and sanitation goods, Polish and Other Sanitation Good Manufacturing, Disinfectants & Toilet Bowl Cleaners, Usage, Prevention, Microbial mats, Bacterial infections, Disinfection and disinfectants, Disinfectants

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Characterization of the zinc-containing metalloprotease encoded by zpx and development of a species-specific detection method for Enterobacter sakazakii

Article Abstract:

The characterization of zinc-metalloprotease of Enterobacter sakazakii, identification of protease gene locus, zpx and development of a species-specific PCR detection assay for Enterobacter sakazakii are discussed. PCR analysis revealed that Enterobacter sakazakii strains possessed the metalloprotease gene, zpx and the deduced amino acid sequence of the metalloprotease has 341 amino acid residues.

author: Kothary, M.H., Tall, B.D., McCardell, B.A., Frazar, C.D., Deer, D.
Analysis, Metalloproteins, Amino acid sequence, Amino acid sequencing

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Retention of virulence in a viable but nonculturable Edwardsiella tarda isolate

Article Abstract:

A study was conducted to investigate the viable but nonculturable (VBNC) state and virulence retention of Edwardsiella tarda bacterium. The results show that when the cells entered into the VBNC state, they gradually changed in shape from short rods to coccoid and decreased in size, but the resuscitative cells did not show any obvious differences from the normal cells.

author: Yun Li, Meng Du, Jixiang Chen, Xiaohua Zhang, Aijuan Li, Yingeng Wang
Bacterial growth, Virulence (Microbiology)

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subjects list: Research, Physiological aspects, Enterobacter, Enterobacteriaceae
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