Effect of tungstate on nitrate reduction by the hyperthermophilic archaeon Pyrobaculum aerophilum

Article Abstract:

Research was conducted to examine the effect of tungstate on nitrate reduction by the hyperthermophilic archaeon Pyrobaculum aerophilum. Growth studies using various tungstate concentrations were carried out to investigate the influence of tungstate on the ability of P. aerophilum to respire anaerobically with nitrate. Results demonstrate that P. aerophilum requires the metal oxyanion WO(sub 4)(super 2-) for its anaerobic growth on yeast extract, peptone, and nitrate as carbon and energy sources.

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Enzymological characteristics of the hyperthermostable NAD-dependent glutamate dehydrogenase from the archaeon Pyrobaculum islandicum and effects of denaturants and organic solvents

Article Abstract:

A study was conducted to examine glutamate dehydrogenase distribution in hyperthermophilic archaea. A Shimadzu 160A spectrophotometer was utilized to assay enzyme activities while glutamate dehydrogenase was purified by Pyrobaculum islandicum at room temperature. The ability of enzymes to catalyze the oxidative deamination of various alpha-amino acids and the reduction amination of various 2-oxo acids was also investigated.

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Reduction of Fe(III), Mn(IV), and toxic metals at 100 degrees C. by Pyrobaculum islandicum

Article Abstract:

Certain hyperthermophilic microorganisms could be used to reduce toxic metals in contaminated water or waste streams. Pyrobaculum islandicum can reduce Fe(III) and other metals using hydrogen, peptone, or yeast extract as electron donors.

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