Effect of pH and monensin on glucose transport by Fibrobacter succinogenes, a cellulolytic ruminal bacterium

Article Abstract:

Fibrobacter succinogenes cells utilize glucose via a pH-sensitive, sodium symport that requires either an electrical or a chemical sodium gradient. However, a pH of less than 5.5 can inhibit glucose uptake despite the presence of a gradient. These conclusions were based on studies using cells of F. succinogenes strain S85 which revealed inhibition of the glucose carrier at a low pH. The ionophores monesin and lasalocid also prevent glucose transport by decreasing intracellular ATP resulting in dissipation of both the electrical and chemical gradients of sodium.

author: Chow, Jo May, Russel, James B.

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Metabolism of phosphonoacetate as the sole carbon and phosphorus source by an environmental bacterial isolate

Article Abstract:

A gram-negative bacteria capable of capable of metabolizing organophosphate was isolated from the environment. The isolate, designated 23F, can use phosphonoacetate as its sole carbon and phosphorus source. Phosphonoacetate utilization is thought to involve cleavage of the C-P bond of organophosphates by C-P lyase. However, failure to detect potential products arising from such a mechanism makes the pathway of phosphonoacetate degradation still uncertain.

author: McMullan, G., Harrington, F., Quinn, J.P.
Analysis, Organophosphorus compounds, Organic phosphorus compounds

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Influence of oxygen and glucose on primary metabolism and astaxanthin production by Phaffia rhodozyma in batch and fed-batch cultures: kinetic and stoichiometric analysis

Article Abstract:

The effects of oxygen and glucose supply on Phaffia rhodozyma metabolism and astaxanthin synthesis are investigated to determine the kinetics and stoichiometry of the reaction. Results indicate that astaxanthin production is affected by anabolism, respiration and fermentation in P. rhodozyma. It is necessary to optimize that whole metabolic pathway in the cell and the synthetic pathway for astaxanthin in P. rhodozyma to increase astaxanthin production.

author: Kakizono, Toshihide, Yamane, Yu-Ichi, Higashida, Katsuya, Nakashimada, Yutaka, Nishio, Naomichi
Microbiological synthesis

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subjects list: Research, Gram-negative bacteria, Microbial metabolism
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