Effect of culture conditions on ergosterol as an indicator of biomass in the aquatic hyphomycetes

Article Abstract:

The ergosterol content of fungi can vary by a factor of 14 depending on the culture medium, oxygen availability, and other growth conditions. Therefore researchers should be cautious when using ergosterol content to estimate fungal biomass.

author: Charcosset, Jean-Yves, Chauvet, Eric
Measurement, Growth, Fungi

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Ergosterol-to-biomass conversion factors for aquatic hyphomycetes

Article Abstract:

The detemination ofergosterol concentrations in the mycelium of aquatic hyphomycete species commonon decaying leaves in running waters is discussed. Ergosterol was extracted from single spore isolates of aquatic hyphomycetes on mineral salt solution, malt extract broth and leaf extract broth and tested for effects of fungal species or strain, medium composition and culture age by one- and two-way analyses of variance. Results indicate that general conversion factor can estimate fungal biomass associated with leaf litter in streams.

author: Chauvet, Eric, Gessner, Mark O.
Analysis

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Temperature and sporulation of aquatic hyphomycetes

Article Abstract:

Research was conducted to determine the effect of temperature on the sporulation of eight common aquatic hyphomycetes grown on yellow poplar leaves in stream-simulating microcosms. The hyphomycetes displayed differences in the number of conidia generated from a similar amount of leaf at the same temperature. Six species showed the same temperature responses for growth on malt extract agar while two species produced the same numbers of conidia at 20 and 25 degrees C. Results provide evidence that aquatic hyphomycetes afford similar amounts of resources to reproduction.

author: Chauvet, Eric, Suberkropp, Keller
Research, Aquatic microbiology, Microorganisms, Temperature effects

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subjects list: Biomass
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