Effect of L-proline on sake brewing and ethanol stress in Saccharomyces cerevisiae

Article Abstract:

The role of intracellular L-proline in cells of Saccharomyces cerevisiae grown under ethanol stress is evaluated. The strain that could accumulate L-proline was used to brew sake containing five times more L-proline than what is found in sake crewed with the control strain, without affecting the fermentation profiles.

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Gene dosage effect of L-proline biosynthetic enzymes on L-proline accumulation and freeze tolerance in Saccharomyces cerevisiae

Article Abstract:

The gene dosage effect of three L-proline biosynthetic enzymes on L-proline accumulation and freeze tolerance in a non-L-proline-utilizing strain is examined. Overexpression of the wild-type enzymes has no influence on L-proline accumulation, which suggests that the complex is very unstable in nature.

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Desensitization of feedback inhibition of the Saccharomyces cervisiae [gamma]-glutamyl kinase enhances proline accumulation and freezing tolerance

Article Abstract:

Enzymatic analysis of recombinant proteins was carried out and Saccharomyces cervisiae was subjected to feedback inhibition by proline. It was seen that yeast cell expressing mutant [gamma]-glutamyl kinase were more tolerant to freezing stress than cells expressing the Asp154Asn mutant.

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