Article Abstract:
The addition of the reductive acetogen Peptostreptococcus productus ATCC 35244 and 2-bromoethanesulfonic acid (BES) promotes the production of acetic acid and the consumption of H2 in the ruminal ecosystem. However, this reductive acetogenesis is stimulated only when methanogenesis is selectively suppressed. P. productus ferments C6 units heterotrophically to acetate. The addition of P. productus to BES-treated samples suppresses acetic acid production and promotes volatile fatty acids production. This leads to a gain of 13 to 15% of energy gain.
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Article Abstract:
Beta-liase mediate the deconjugation and nitroreduction of 1-nitropyrene (1-NP) 9,10-oxide-cysteine which enhance the mutagenicity, tumorigenicity, and absorbability of 1-NP. Beta-liase increases the DNA binding capacity of 1-NP which is an environmental pollutant and causes tumors. The optimal pH for beta-liase activity purified from Peptostreptococcus magnus lies between 7.5 and 8.0. It is widely distributed in intestinal bacteria and occurs in mammalian livers and kidneys.
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Article Abstract:
A strain of Escherichia coli was transformed using a plasmid in which the gene coding for serine acetyltransferase in the cysE group of genes was altered by site-directed mutagenesis to remove the feedback inhibition of the enzyme by L-cysteine. The transformants were able to produce significant quantities pf L-cysteine and L-cystine although the production was erratic because the host has a cysteine-degrading enzyme.
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