Article Abstract:
Research was conducted to investigate the synthesis of a de novo balanced translocation in a patient with X-linked lissencephaly and double cortex syndrome. Cosmids were selected for shotgun sequencing while polymerase chain reactions were performed on a nondenaturing acrylamide gel run. DNA sequencing was also performed in study using generalized techniques via an automated sequencer. Results of the study showed that the translocation characterized a novel predicted protein called Doublecortin.
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Article Abstract:
Purified mammalian and yeast Sec61p complexes in detergent form cylindrical oligomers with a diameter of approximately 85 angstroms and a central pore of approximately 20 angstroms. These cylindrical oligomers are proposed to represent protein-conducting channels of the endoplasmic reticulum membrane. Through electron microscopy, it was found that each oligomer contains 3-4 heterotrimers. The Sec61p complex's association with ribosomes or the Sec62/63p complex stimulates its oligomer formation.
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Article Abstract:
Research was conducted to determine the location and orientation of elongation factor G (EF-G) in the ribosome utilizing directed hydroxyl radical probing to understand the mechanism of EF-G-catalyzed translocation. Polymerase chain reaction was utilized to amplify the EF-G gene from the genomic DNA of Escherichia coli. Results showed that two of the rRNA targets were conserved parts of 23S rRNA that played a major role in elongation factor-dependent functions.
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