Article Abstract:
A variety of opine-catabolizing pathogenic Agrobacterium and Pseudomonas bacteria are present in the crown galls of plants such as grape, plum and blackberry. An analysis of 43 crown galls shows that the most abundant opine is nopaline while octopine is absent. The tumors of wild blackberry contain an unidentified iminodiacid of the succinamopine-leucinopine type. Plum and cherry tumors show a good relation between the presence of opine and opine-using bacteria. However, apple and plum tumors contain the bacteria but no opine. Some tumors also contain opine-using nonpathogenic bacteria.
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Article Abstract:
The detection of all pathogenic Agrobacterium strains by PCR may be possible using a primer pair derived from the conserved endonuclease domain of VirD2 protein. In addition, a primer pair derived from the cytokinin synthesis gene ipt allowed A. tumefaciens to be distinguished from A. rhizogenes, demonstrating the ability to distinguish tumor-inducing from root-inducing strains. Both primer pairs could be used in the same PCR reaction without interference.
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Article Abstract:
The physiological chrarcterizationof Streptomyces isolates which included Israeli potato scab-inducing strains and some American reference strains of Streptomyces scabies is discussed. Strain similarities were determined by numerical taxonomy and were classified by restriction fragment length polymorphism. It was found that a phenotypicallyand genetically diverse population of Streptomyces strains causes potato scab in Israel.
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