Identification of cell wall proteins of Bacteroides fragilis to which bacteriophage B40-8 binds specifically

Article Abstract:

Cell wall proteins of Bacteroides fragilis to which bacteriophage B40-8 binds specifically have been identified for the first time. The bacteriophages have been proposed as indicators of virological fecal pollution. The lytic bacteriophage B40-8 has been used as a model for studying interactions of phages with B. fragilis.

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Culture and decontamination methods affecting enumeration of phages infecting Bacteroides fragilis in sewage

Article Abstract:

The use of a new medium called Bacteroides phage recovery medium in the culture of phages affecting B. fragilis permitted faster bacterial growth and produced higher phage yields. The recovery of phages was higher with the use of polyvinylidene diflouride membranes. Although eluting treatments may increase the yield of phages, pretreatment of the water with glycine did not significantly increase phage recovery.

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Diversity of Bacteroides fragilis strains in their capacity to recover phages from human and animal wastes and form fecally polluted wastewater

Article Abstract:

A study was conducted to identify host strains of Bacteroides fragilis other than HSP40 that has the capability to detect bacteriophages of human and animal feces from urban sewages. Six strains were identified and tested. Results suggest that strain RYC4023, like HSP40, can only detect phages from human feces. On the other hand, the other four strains of Bacteriodes fragilis were observed to be capable of finding phages in the feces of animal species and in wastewater polluted with both human and animal feces. Strain RYC2056 was noted to detect the most number of bacteriophages.

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