Photosynthetic and phylogenetic primers for detection of anoxygenic phototrophs in natural environments

Article Abstract:

A PCR primer that targets the pufM gene in purple phototrophic bacteria is capable of amplifying DNA from purple sulfur phototrophs, purple nonsulfur phototrophs, and Chloroflexus species. The pufM gene codes for the M subunit of the photosynthetic reaction center, which is universal among purple phototrophic bacteria. The primer did not amplify cyanobacterial DNA despite the similarity between the purple bacterial reaction center and photosytem II in green plants.

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Diversity and distribution of sulfate-reducing bacteria in permanently frozen Lake Fryxell, McMurdo Dry Valleys, Antarctica

Article Abstract:

The populations of sulfate-reducing bacteria in Lake Fryxell, both 16 rRNA gene and metabolic primer sets targeting the dsrA gene for the dissimilatory sulfite reductase alpha subunit were employed to analyze environmental DNA obtained from the water column and sediments of Lake Fryxell were investigated. Several clone groups were highly localized with respect to lake depth and therefore experienced specific physiochemical conditions.

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Biodiversity of methanogenic and other archaea in the permanently frozen Lake Fryxell, Antarctica

Article Abstract:

The biodiversity of Archaea in the permanently frozen Lake Fryxell in the Antarctica is explored. Two clusters of methanogens were detected in the sediments, and another cluster of possibly methanotrophic Euryarchaeota was detected in the anoxic water column just above the sediments.

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