Article Abstract:
A research was conducted to investigate the metabolic activity of Pseudomonas fluorescens DF57 in the barley rhizosphere and to test whether sufficient phosphate was available to the bacterium. A Hamamatsu photonic camera system was utilized to characterize bioluminescence from colonies and colonized roots. Results showed that the average cell-specific light emission of DF57-40E7 varied among different locations in the rhizosphere and that the rhizoplane population of DF57-40E7 exhibited the lowest cell activity at the root tip.
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Article Abstract:
Sodium dodecyl sulfate-polyacrilamide gel electrophoresis (SDS-PAGE) was utilized for the separation and analysis of Pseudomonas fluorescens Ag1 phosphate-starvation-inducible outer membrane protein. SDS-PAGE analysis of Pseudomonas fluorescens Ag1 indicated the presence of an novel N-terminal amino acid sequence termed, Psi1. Furthermore, the Psi1 N-terminal amino acid sequence of Ag1 did not react with Psi1 antibodies in intact phosphate-starved Pseudomonas fluorescens cells.
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Article Abstract:
The outer membrane protein profiles of Pseudomonas fluorescens and P. putida are variable and cannot be used to identify the specific biovar of different strains. However the OprE, OprF, OprH and OprL proteins have been conserved in the strains. The purified OprF raises a polyclonal antibody that reacts with the OprF proteins from other Pseudomonas rRNA homology group 1 proteins. The antibody does not react with proteins from nonpseudomonads.
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