Distinguishing the effects of mutational biases and natural selection on DNA sequence variation

Article Abstract:

A clarification of the distinction between mutational and natural selection biases is attempted while proposing some techniques of differentiating between changes in mutation patterns and natural selection actions. The mutation configurations of DNA sequences will not differ if four assumptions are met. However, Adam Eyre-Walker's assumption has differed from the third assumption. Comparing base composition evolution and mutation evolutionary dynamics are two methods to distinguish between selection and mutation biases when interpreting departures from the four assumptions.

author: Akashi, Hiroshi
Natural selection

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Sodium orthovanadate-resistant mutants of Saccharomyces cerevisiae show defects in golgi-mediated protein glycosylation, sporulation and detergent resistance

Article Abstract:

Resistance to orthovanadate, which is very quick in Saccharomyces cerevisiae, produces mutants that exhibit defects in glycosylation, sporulation and low viability in liquid culture. Vanadium is essential for eukaryotic cell growth, and the biologically active orthovanadate in eukaryotic cells is similar in structure to orthophosphate. Oxyanions such as orthovanadate and arsenate are toxic to cells as they compete with phosphate or sulfate. Vanadium exists in a number of oxidation states in cells and orthovanadate polymerizes at low pH.

author: Montalvo, Ermelinda, Neff, Norma, Kanik-Ennulat, Cynthia
Usage, Vanadium

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Removal of a Mig1p binding site converts a MAL63 constitutive mutant derived by interchromosomal gene conversion to glucose insensitivity

Article Abstract:

The yeast Saccharomyces cerevisiae has several strains capable of fermenting maltose because of the presence of several complex loci called MAL. MAL43c is one of the alleles of the three genes contained in MAL and is insensitive to glucose repression. Through gene conversion, an allele which is sensitive to glucose repression, MAL63c, was made to exhibit characteristics similar to MAL43c. The process involved the removal of a Mig1p binding site from MAL63c.

author: Wang, Jianfan, Needleman, Richard
Gene expression

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subjects list: Analysis, Mutation (Biology), Mutation, Research, Saccharomyces
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