Disruption of mouse RAD54 reduces ionizing radiation resistance and homologous recombination

Article Abstract:

An analysis was conducted of the phenotype of mouse RAD54-l- cells. These cells are sensitive to mitomycin C, ionizing radiation and methyl methanesulfonate but not to ultraviolet light. Gene targeting studies indicate that homologous recombination in mRAD54-l- cells is less compared to wild-type cells. The findings suggest that mRAD54 is not required for the recombination processes that generate functional immunoglobulin and T cell receptor genes.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Bcl-2 can rescue T lymphocyte dvelopment in interleukin-7 receptor-deficient mice but not in mutant rag-1(super -l-) mice

Article Abstract:

A deficiency in interleukin-7 (IL-7) receptor among mice causes them to be lymphopenic since the occurrence of defects in cell expansion becomes greater. As a result, the few mature T cells that develop in IL-7R(super -l-) animals are functionally impaired. However, the overexpression of antiapoptosis protein Bcl-2 resolves these defects by blocking positive signals resulting to the death of impaired T cells.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Nicholas (Nick) R. Cozzarelli: 1938-2006

Article Abstract:

Nicholas R. Cozzarelli, professor of cell and molecular biology at the University of California, Berkeley, was a mechanistic biochemist by training. He spent his whole research career working and thinking about DNA.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: