Disruption of a glycogen-branching enzyme gene, glgB, specifically affects the sporulation-associated phase of glycogen accumulation in Streptomyces aureofaciens

Article Abstract:

Streptomyces aureofaciens cells containing a mutated glgB gene show normal accumulation of glycogen during aerial mycelium formation (phase I) but defective accumulation during sporulation (phase II). The glgB gene encodes a glycogen-branching enzyme that controls the last stage of glycogen synthesis. The mutants show normal sporulation but contain only unbranched glucan during sporulation. The mutants contain one glycogen-branching enzyme while the wild type cells contain two. This indicates that S. aureofaciens contains two genes that regulate phase I and phase II glycogen accumulation.

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Proteomic analyses of a Listeria monocytogenes mutant lacking [[sigma].sup.B] identify new components of the [[sigma].sup.B] regulon and highlight a role for [[sigma].sup.B] in the utilization of glycerol

Article Abstract:

A proteomic approach is used for defining the components of the [[sigma].sup.B] regulon in Listeria monocytogenes 10403S (serotype 1/2a), which have played vital roles in both virulence and stress tolerance. Reverse transcription-PCR (RT-PCR) analysis of [[sigma].sup.B]-dependent genes along with proteomic analyses have shown that [[sigma].sup.B] has played a major role in the stationary phase of growth in complex media.

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Cultivation system using glass beads immersed in liquid medium facilitates studies of Streptomyces differentiation

Article Abstract:

A two-phase cultivation system was developed which enables studies of streptomycete differentiation by molecular biological and global techniques such as transcriptomics and proteomics. This system permits easy manipulation of medium composition during differentiation of Streptomyces.

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