Disruption of TRI101, the gene encoding trichothecene 3-O-acetyltransferase, from Fusarium sporotrichioides

Article Abstract:

In order to determine whether or not inactivation of TRI101, the gene encoding trichothecene 3-O-acetyltransferase in Fusarium sporotrichioides, is lethal and whether or not TRI101 acts in both Fusarium self-protection against trichothecenes and trichothecene biosynthesis, a Fusarium sporotrichioides NRRL 3299 cDNA expression library has been screened in a toxin-sensitive Saccharomyces cerevisiae strain with no functional PDR5 gene. Disruption of TRI101 was not lethal, and deletion mutants accumulated isotrichodermol, a 3-hydroxytrichothecene. TRI101 converts isotrichodermol to isotrichodermin and is necessary for biosynthesis of T-2 toxin. Trichothecenes are antibiotics. Their biosynthesis would likely require special protective adaptation by organisms that produce them.

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Tri16 is required for esterification of position C-8 during trichothecene mycotoxin production by Fusarium sporotrichioides

Article Abstract:

Research indicates that Tri16 encoded C-8 acyltransferase mediates T-2 production by Fusarium sporotrichioides. The additional trichothecene gene cluster comprising Tri16 and Tri1 add the C-8 substituents during the production of the toxic trichothecenes. Data reveal that the acyltransferase catlyzes ester side group formation at C-8 during trichothecene biosynthesis.

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Cloning of a gene encoding an Alt a 1 isoallergen differentially expressed by the necrotrophic fungus Alternaria brassicicola during Arabidopsis infection

Article Abstract:

A new isoallergen Alt b 1 is reported from the fungus Alternaria brassicicola. A 92% sequence homology between Alt a 1 and Alt b 1 suggests that the latter can be treated as an isoallergen. Data indicate that the new isoallergen is differentially expressed during A. brassicicola pathogenesis on Arabidopsis.

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