Discriminant analysis of ribotype profiles of Escherichia coli for differentiating human and nonhuman sources of fecal pollution

Article Abstract:

Escherichia coli is a widespread bacterium in the intestines of warm-blooded animals and is employed as an indicator of fecal pollution. A total of 238 E. coli isolates from human sources (HS) and nonhuman sources (NHS) were gathered from the Apalachicola National Estuarine Research Reserve, from associated sewage treatment plants and directly from animals and tested for ribotype profile. It was observed that discriminant analysis of ribotype profiles may be a useful technique for identifying HS and NHS fecal pollution.

author: Tamplin, Mark L., Portier, Kenneth M., Parveen, Salina, Edmiston, Lee, Robinson, Kevin
Escherichia coli, Water pollution

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Tauroconjugation of cholic acid stimulates 7-alpha-dehyroxylation by fecal bacteria

Article Abstract:

The conjugation of taurine with cholic acid (CA) increases the 7-alpha-dehydroxylation of taurocholic acid (TCA) in fecal, defined limited fecal, and binary bacterial cultures. The dehydroxylation in fecal and limited fecal cultures is unaffected by free taurine but increases in binary cultures. The reduction of taurine to H2S is necessary for the activity of TCA. The minimal flora necessary for the dehydroxylation is the Bacteroides sp. strain R1 and Clostridium sp. strain 9/1.

author: Eldere, J. van, Celis, P., Pauw, G. de, Lesaffre, E., Eyssen, H.
Physiological aspects, Cholic acid, Taurine

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Persistence of PCR-detectable Bacteroides distasonis from human feces in river water

Article Abstract:

A study was conducted to analyze the persistence of polymerase chain reaction-detectable Bacteroides distasonis in surface water. Human feces were dispersed into water and incubated in flasks to carry out the analysis. In filtered water, an eukaryotic inhibitor was extended by at least seven days. Results indicated that the persistence of PCR-detectable DNA from the bacterium is influenced by temperature and predation.

author: Kreader, Carol A.
Analysis, DNA, Bacteria, Water, Water analysis

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subjects list: Research, Feces, Microbiology
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