Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and alpha-amylase

Article Abstract:

Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis alpha-amylase. This is done by using the C-terminal-half region of alpha-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins.

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Construction of a xylan-fermenting yeast strain through codisplay of xylanolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells

Article Abstract:

The construction of a yeast strain that hydrolyzes birchwood xylan to xylose is undertaken by codisplaying Trichoderma reesei xylanase II (XYNII) and Aspergillus oryzae beta-xylosidase (XylA) on the cell surface of Saccharomyces cerevisiae. The results show that the direct conversion of xylan to ethanol is accomplished by the xylan-utilizing S. cerevisiae strain.

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Synergistic saccharification and direct fermentation to ethanol, of amorphous cellulose by use of engineered yeast strain codisplaying three types of cellulolytic enzyme

Article Abstract:

Sustainable energy resource in the world and ethanol produced from biomass is the most widely used biofuel when blended with gasoline. A whole cell biocatalyst, which has the ability to induce synergistic as well as sequential cellulose-degradation reaction was constructed, through codisplay of three types of cellulolytic enzymes.

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