Direct labeling of polyphosphate at the ultrasound level in Saccharomyces cerevisiae by using the affinity of the polyphosphate binding domain of Escherichia coli exopolyphosphatase

Article Abstract:

A novel technique is developed using the affinity of the polyphosphate binding domain of Escherichia coli exopolyphosphatase to polyphosphate (polyP) to investigate polyP localization in Saccharomyces cerevisiae. The localization of polyP at the electron microscopic level for the first time is demonstrated and the visualization of poly localization with much higher specificity and resolution than with other conventional methods is enabled.

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Prolonged maltose-limited cultivation of Saccharomyces cerevisiae selects for cells with improved maltose affinity and hypersensitivity

Article Abstract:

The effect of prolonged maltose-limited chemostat cultivation on the physiology of Saccharomyces cerevisiae is examined by monitoring the affinity of maltose, genome-wide transcript levels, activities of key enzymes and the physiological responses to maltose excess during the cultivation in maltose-limited cultures. The results show that in some cases the selection may take place even at the expense of the microbial performance.

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Physiological and transcriptional responses of Saccharomyces cerevisiae to zinc limitation in chemostat cultures

Article Abstract:

The studies of transcriptional responses of the yeast Saccharomyces cerevisiae mutant strain to Zn availability in chemostat culture are reported. A Zn-specific Zap1p regulon, consisting of 26 genes is identified as Zn-dependent mediator responsible for carbohydrate storage metabolism and mitochondrial biogenesis.

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