Article Abstract:
The adhesion of Azospirillum brasilense to inert surfaces such as glass and polystyrene was studied to determine the function of compounds produced by the cells. The morphology of the adhering cells was analyzed by scanning electron microscopy. The amount of protein at the surface of a cell sediment increased as a function of time at 30 degrees C, correlating with an increase of adhesion density. A similar correlation between protein amount and adhesion density was found when contrasting exponential-phase cells with stationary-phase cells.
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Article Abstract:
The flagellin of the polar flagellum of the gram-negative bacterium, Azospirillum brasilense Sp7, undergoes glycosylation. The glycosylation is verified by the reduction in apparent Mr after chemical deglycosylation, by one-dimensional gel electrophoresis followed by sugar staining and by combined utilization of a sugar-specific monoclonal antibody in immunogold labelling and transmission electron microscopy. Two mutant flagellins varying in glycan composition with respect to the wild-type are analyzed.
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Article Abstract:
Azospirillum bacteria are able to colonize plant roots. Azospirllum mutants have been investigated, using the beta-glucoronidase (GUS) reporter system, to determine their capacity to initiate wheat root colonization at the root hair zones. Non-flagellated mutants and a non-chemotactic mutant showed strong colonization reduction ability.
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