Article Abstract:
A simple yet fast procedure by means of the reverse transcriptase-polymerase chain reaction (RT-PCR) approach was developed to detect viable bacteria, molds, and yeasts in milk samples after treatment. The RT-PCR method proved to be a potent tool in detecting viable contaminants because of its high potential to increase detection sensitivities and speed, with the detection level placed at 10 cells ml of milk(super -1). Because of its rapidity and simplicity, it can be easily transposed to other foods and other contaminants.
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Article Abstract:
A new polymerase chain reaction method for the direct detection of Clostridium tyrobutyricum in raw milk provides sensitive and reliable results in less time than the immunological approach. The bacterial spores are isolated by chemical extraction and centrifugation. The method is independent of the germination efficiency of spores and produces the result before cheese is formed from the milk. A two step PCR amplification procedure with two nested pairs of primers is required for spore detection in raw milk.
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Article Abstract:
A new method of detecting Bacillus sporothermodurans in raw milk using polymerase chain reaction is presented. B. sporothermodurans is a gram-positive bacterium with endospores that are resistant to sterilization and ultrahigh temperature treatment of raw milk. The method relies on subtractive hybridization to obtain specific sequences of the bacterium's DNA. The detection limits are 9, 0.4 and 0.22 CFU/ml. for 1, 10, and 100 ml. of raw milk.
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