Direct 5S rRNA assay for monitoring mixed-culture bioprocesses

Article Abstract:

A direct 5S rRNA assay technique is useful in separating rRNA of bacteria in acidic environments and in mixed-culture ore bioprocesses. The technique uses high-resolution denaturing gradient gel electrophoresis to separate 5S rRNA isolated from biomass. Separation is based on differences in migration characteristics of 5S rRNA during gradient gel electrophoresis. The method gives a powerful resolution at the species level. The assay is useful in distinguishing between closely related species such as Thiobacillus ferrooxidans ATCC 19859 and Thiobacillus thiooxidans ATCC 8085.

author: Stoner, D.L., Browning, C.K., Bulmer, D.K., Ward, T.E., MacDonell, M.T.
Methods, Gel electrophoresis, Ribosomal RNA, Isolating mechanisms, Microbiological assay

User Contributions:

Comment about this article or add new information about this topic:

CAPTCHA

Application of stereolithographic custom models for studying the impact of biofilms and mineral precipitation on fluid flow

Article Abstract:

The application of stereolithographic models and the integration of three-dimensional lattice Boltzmann simulations to microbiological studies are reported. Several models with a variety of internal configurations are designed, fabricated and used to examine the impact of biomass accumulation and microbially induced calcite precipitation on fluid flow.

author: Stoner, D.L., Watson, S.M., Stedfeld, R.D., Meakin, P., Griffel, L.K., Tyler, T.L., Pegram, L.M., Barnes, J.M., Deason, V.A.
United States, Microbiological synthesis, Biomass energy

User Contributions:

Comment about this article or add new information about this topic:

CAPTCHA

Does green light influence the fluorescence properties and structure of phototrophic biofilms?

Article Abstract:

The effects of illuminating biofilms formed by Gloeothece membranacea (cyanobacteria) and Chlorella sorokiniana (Chlorophyta) using exclusively white or green light is compared and evaluated. Conclusion suggests that green light could prevent the growth of photosynthetic organisms, expect for those capable of modifying accessory pigments.

author: Roldan M., Oliva, F., Gonzalez del Valle, M.A., Saiz-Jimenez, C., Hernandez-Marine, M.
Cyanobacteria, Fluorescence

User Contributions:

Comment about this article or add new information about this topic:

CAPTCHA


subjects list: Research, Analysis, Microbial mats
This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.