Article Abstract:
A modified randomly amplified polymorphic DNA (RAPD) procedure was used in differentiating seven dextran-producing Leuconostoc strains. The latter are lactic acid bacteria that produce extracellular polymers such as altneran, dextran and levan from sucrose metabolism. The RAPD method uses specific primers culled from conserved regions of the dextransucrase genes. It is a simpler approach than other methods that require time-consuming polymer structural analyses.
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Article Abstract:
The proteins LcaC and LcaD may play a crucial role in the production of the antibacterial protein leucocin A produced by Leuconostoc gelidum UAL187. When inserting DNA into cells that did not normally produce leucocin A, the genes for LcaC and LcaD were necessary to stimulate the production of leucocin A. The genes for LcaC and LcaD were located upstream and on the opposite DNA strand from the stuctural gene for leucocin A.
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Article Abstract:
A study was conducted to study the Leloir pathway from Lactobacillus casei 64H and to characterize the cloning and sequencing of the major part of the gal operon of L. casei. 64H was maintained in calcium carbonate-fortified litmus milk with glucose while chromosomal DNA was determined from L. casei. Results showed that galR is transcribed on policistronic messengers indicating that operons may be autoregulated.
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