Differentiation of chitinase-active and non-chitinase-active subpopulations of a marien bacterium during chitin degradation

Article Abstract:

Researchers describe two subpopulations of Pseudoalteromonas sp. strain S91, one that can degrade chitin and one that cannot degrade chitin but can live off chitin degradation products released by the other organism. This study utilized thin films of pure, spun-cast chitin and an enzyme-linked fluorescent probe to detect bacterial chitinase activity.

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Spatial and temporal variations in chitinolytic gene expression and bacterial biomass production during chitin degradation

Article Abstract:

Only chitin-degrading marine bacteria that grow directly on chitin produce chitinolytic enzymes. Cells that subsequently detach and become part of the free-living population have inactive chitin-degrading genes.

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Use of green fluorescent protein to tag and investigate gene expression in marine bacteria

Article Abstract:

Gene expression in marine bacteria was studied by comparing the levels of expression of gfp-mut from two different bacterial promoters on a broad-host range plasmid in three gram-negative marine bacterial species. Initial biofilm formation on a natural biodegradable substratum was studied using the green fluorescent protein-tagged S91 cells. The hydrated structure of the biofilm was examined using laser scanning confocal microscopy.

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