Differences in hyporheic-zone microbial community structure along a heavy-metal contamination gradient

Article Abstract:

Research has been conducted on relationship between hyporheic microbial community structure and fluvially deposited heavy metal concentration in river sediments. The authors describe the study of this relationship carried out via the use of denaturing gradient gel electrophoresis, 16S rRNA gene phylogenetic analysis, quantitative PCR and phospholipid fatty acid analysis.

author: Holben, William E., Feris, Kevin, Ramsey, Philip, Frazar, Chris, Moore, Johnnie N., Gannon, James E.
Analysis, Polymerase chain reaction, Gel electrophoresis, Phospholipids, River sediments, Contamination (Technology), Contamination, Heavy metals, Structure

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Expression of a cloned cyclopropane fatty acid synthase gene reduced solvent formation in Clostridium acetobutylicum ATCC 824

Article Abstract:

Research has been conducted on cyclopropane fatty acid synthase gene from Clostridium acetobutylicum ATCC 824. The cloning and expression of this gene have been carried out under clostridial ptb promoter control, and the results are discussed.

author: Bennett, George N., Rudolph, Frederick B., Zhao, Yinsuo, Hindorff, Lucia A., Chuang, Amy, Monroe-Augustus, Melanie, Lyristis, Michael, Harrison, Mary L.
Cloning, Cyclic compounds, Propane

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Overexpression of groESL in Clostridium acetobutylicum results in increased solvent production and tolerance, prolonged metabolism, amd changes in the cell's transcriptional program

Article Abstract:

The authors have investigated Clostridium acetobutylicum ATCC 824. They describe the use of DNA arrays and Western blot analyses in studying role of groESL overexpression and host-plasmid interactions in this bacterium.

author: Papoutsakis, Eleftherios T., Tomas, Christopher A., Welker, Neil E.
Cells (Biology), Metabolism, Genetic transcription, Operons, Transcription (Genetics), Cells, DNA microarrays, Western immunoblotting, Western blot

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subjects list: Research, United States, Usage, Physiological aspects, Environmental aspects, Genetic aspects, Microbiology, Fatty acids, Microbial populations, Microbial ecology, Clostridium, Gene expression, Solvents
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