Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR
Article Abstract:
A study has revealed that DNA sequences in Salmonella rfb operons have identified regions that are conserved and regions that are unique to a given Salmonella serovar. The conservation of these rfb gene sequences were believed to be reflective of the presence of common sugars on O antigens in some members of the Enterobacteriaceae family. A polymerase chain reaction test conducted on Escherichia coli O157 serotypes revealed that as few as 10 cells with the specific rfb oligonucleotide primers were present.
author: Sanchez, Susan, Maurer, John J., Lee, Margie D., Schmidt, Denise, Petrosko, Patricia, Bolton, Lance
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
Escherichia coli, Bacterial antigens, O antigens
Dissemination of fluoroquinolone-resistant Campylobacter spp. within an integrated commercial poultry production system
Article Abstract:
Commercial broiler hatching eggs, which were incubated in a research facility until the chicks hatched, were obtained to determine whether the presence of Campylobacter DNA in young chicks was due to ingestion of the bacteria in food or water. The results have shown that resistant Campylobacter coli can be present in multiple levels of an integrated poultry system and molecular methods or more sensitive culture techniques might be required to detect early colonization by Campylobacter in broiler chicks.
author: Hofacre, Charles L., Sanchez, Susan, Maurer, John J., Idris, Umelaalim, Lee, Margie D., Jingrang Lu, Maier, Marie, Harmon, Barry G.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
Pharmaceutical Preparation Manufacturing, Pharmaceutical preparations, Quinolone Antibiotics, Broiler, fryer, and roaster chickens, Chickens, Broilers, Broilers and Other Meat Type Chicken Production, Diseases, Drug resistance in microorganisms, Microbial drug resistance, Quinolone antibacterial agents, Fluoroquinolones, Broilers (Poultry), Broilers (Chickens), Quinolones, Chemical properties
Rapid detection of Campylobacter coli, C. jejuni, and Salmonella enterica on poultry carcasses by using PCR-enzyme-linked immunosorbent assay
Article Abstract:
Research has been conducted on food-borne pathogens. The authors describe the use of multiplex PCR primer set and probes developed on the basis of Campylobacter coli, C. jejuni and Salmonella enterica genes, which encode lipoproteins involved in siderophore transport, to screen poultry carcasses for the food-borne pathogens.
author: Hong, Yang, Berrang, Mark E., Liu, Tongrui, Hofacre, Charles L., Sanchez, Susan, Wang, Lihua, Maurer, John J.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
United States, Enzymes, Other Basic Organic Chemical Manufacturing, Analysis, Causes of, Physiological aspects, Environmental aspects, Foodborne diseases, Microbiology, Salmonella, Lipoproteins, Microbial populations, Contamination (Technology), Contamination, Sorbents, Proteolipids, Immunospecificity, Poultry diseases, Microbial ecology
subjects list: Research, Usage, Polymerase chain reaction, Gene expression, Genetic aspects, Campylobacter
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