Article Abstract:
Culture filtrates and chimeric protein products from apa-2 or ver-1 genes were utilized to develop polyclonal antibodies (PAb) against the aflatoxinogenic strain of Aspergillus parasiticus. The PAbs that were raised against the chimeric protein products of apa-2 and ver-1 genes expressed in Escherichia coli exhibited high specificity for the aflatoxinogenic fungal strain. Furthermore, the antibodies raise against the chimeric fungus proteins exhibited specific immunoreactivity with the native protein of the fungus.
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Article Abstract:
Some species of Fusarium isolated from bananas and grown on autoclaved corn grain, produce mycotoxins toxic to the larvae of Artemia salina. Eight isolates of Fusarium moniliforme, three of F.proliferatum and all isolates of F.camptoceras prove toxic to the A. salina larvae. Fumonisin B1, fumonisin B2, moniliformin, and zearalenone are some of the mycotoxins determined in the corn culture extracts. Additional compounds generated by the fungal isolates are likely to play an important role in the larval toxicity.
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Article Abstract:
A quantitative evaluation of neutralizer toxicity against Acanthamoeba castellanii reveals that the microtiter method gives a lower viable cell count than that given by the hemacytometer method. The Dey-Engley neutralizing medium fails to toxicate A. castellanii and is utilized for testing contact lens disinfection. This accurate technique comparable to the Page's saline method is effective for neutralizing the antimicrobial agents.
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