Article Abstract:
Monoclonal antibodies (MAbs) were developed against Vibrio alginolyticus, V. anguillarum, V. carchariae, V. cholerae, V. damsela, V. furnissii, V. harveyi, V. ordalii, V. parahaemolyticus and V. vulnificus for use in rapid identification of these bacteria. Serological experiments showed that some of the MAbs cross-reacted with closely-related Vibrio species, indicating a common antigenic determinant among them. Three MAbs recognized nearly all of the Vibrio species, which again suggests a common epitope among them. The complementary use of genus-specific and species-specific MAbs is a rapid method for identifying different Vibrio species.
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Article Abstract:
Monoclonal antibodies, B48, B183 and D89 are useful for developing immunological methods for the detection of spores of Bacillus cereus and Clostridium sporogenes. The B48 and B183 react with antigens that are present on the exosporium of the B. cereus T spore. The antibody D89 reacts both with the spores of C. sporogenes and B. cereus, and with the vegetative cells of these genera. This indicates its usefuleness as a detector antibody, once spores are isolated from the food or its ingredients.
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Article Abstract:
Monoclonal antibodies directed against specific antigens of different bacterial taxa were generated for use in the in situ detection of bacteria in their native fresh water environment. Three different bacterial isolates were used to produce the strain-specific monoclonal antibodies. Immunofluorescence microscopy did not show any cross reaction among the monoclonal antibodies generated.
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