Article Abstract:
A species-specific probe for Hyphomicrobium facilis is developed from a transposon Tn5-132 insertion mutant, defective in methanol oxidation, using the inverse polymerase chain reaction method. The probe hybridized only with DNA from H. facilis, its sub species and with some of its strains. The probe is used to identify the presence of H. facilis, by screening the isolated DNA from lawn soil, directly. This screening indicates that H. facilis constitutes 30% of the total hyphomicrobia found in the soil.
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Article Abstract:
A protocol for DNA extraction directly from soil samples uses rDNA of the plant wilt pathogen Verticillium dahliae as the reference sequence. This protocol can further be integrated with polymerase chain reaction (PCR) for DNA-based identification of soil organisms. Samples are ground in liquid nitrogen, suspended in skim milk powder solution, DNA is extracted with sodium dodecyl sulfate buffer-phenol extractant, and collected by ethanol precipitation. DNA thus obtained can be subjected to PCR assay.
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Article Abstract:
Researchers used a newly developed quantitative PCR assay called TaqMan to measure small-subunit rRNA genes from planktonic prokaryotes in Monterey Bay, California. The distribution of planktonic microbes using this method matched the results of other methods, including oligonucleotide probe hybridization, whole-cell hybridization assays, and flow cytometry.
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