Article Abstract:
A chemiluminometric assay detects the beta-galactosidase of coliform bacteria present in water quicker than fluorometry and colorimetry method. One coliform seeded in 100 ml of sterile water can be detected after a 6- to 9-hr propagation phase and a 45-min enzyme assay in the presence of polymixin B. The method can be used to test water samples during emergencies such natural disasters or failure of the water supply.
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Article Abstract:
The enzymatic presence-absence tests fail to detect one waterborne coliform or one Escherichia coli in 100 ml of drinking water. Chemiluminometric procedures reveal that this limitation arises due to a compromise between the specificity of the test and the sensitivity-boosting measures that help to achieve the required speed. Isolation of the target bacteria can improve the specificity and sensitivity of the test.
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Article Abstract:
Milk tetracycline residues were found to inhibit Escherichia coli Beta-galactosidase biosynthesis. The same effect may be produced using other antibacterials, though these have to be in concentrations 10-fold higher. The findings were employed in developing a milk tetracycline screening test based on a Beta-galactosidase chemiluminometric assay. Contaminated samples of raw milk were used to verify the approach.
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