Development of a radioactive protein A-based assay for analysis of surface protein expression in gram-positive bacteria

Article Abstract:

An immunochemical technique which employs radioactive protein A for the detection and analysis of streptococcal M6 protein epitopes on the surface of recombinant Streptococcus gordonii, is shown. Using this assay, S. gordonii cells expressing a portion of the M6 protein on their surfaces exhibited a 75-fold increase in bound radioactivity over cells of the control S. gordonii parental strain. Moreover, this assay indicated that expression is highest at late log phase with the protein being sloughed off during stationary phase.

author: Hruby, Dennis E., Coyle, Tove C., Franke, Christine A.
Research, Streptococcus, Antigenic determinants

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Dose determination for acute salmonella infection in pigs

Article Abstract:

The minimum dose required inducing acute salmonella infection in pigs by intranasal inoculation with Salmonella enterica subsp. entericaserovar Typhimurium is determined and acute Salmonella infection in pigs exposed to a contaminated environment containing various levels of Salmonella is evaluated. The results indicate that intervention against acute Salmonella infection in lairage might be more readily achieved.

author: Harris, D.L., Loynachan, A.T.
United States, Hogs, Hog and Pig Farming, Care and treatment, Swine, Salmonellosis

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Protein folding failure sets high-temperature limit on growth of phage P22 in Salmonella enterica serovar typhimurium

Article Abstract:

The growth limitation at high temperature for Salmonella enterica serovar Typhimurium infected by phage P22 is examined. The results show that the protein folding pathway of the tailspike adhesin sets the high-temperature limit for P22 formation in Salmonella serovar Typhimurium.

author: King, Jonathan, Pope, Welkin H., Hasse-Pettingell, Cameron
Analysis, Causes of, Growth, Protein folding, Salmonella enteritidis, Company growth

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