Development of a genetic transformation system for an alga-lysing bacterium

Article Abstract:

Alteromonas sp. strain A28 that was determined from a coastal seawater lysed a number of algae. The alga-lytic bacteria was prepared using the soft-agar overlayer method while plasmid extractions were carried out by the alkalin lysis technique. Alteromonas cells were determined by electroporation after cells were grown overnight. The efficiency of the chimeric plasmid transformation of A28 was dependent with the source of plasmid DNA.

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Inhibition of quorum sensing in Pseudomonas aeruginosa by N-acyl cyclopentylamides

Article Abstract:

The ability of a series of structural analogs of C8-cyclopentylamide (CPA) to inhibit quorum sensing in Pseudomonas aeruginosa (PAO) is examined. N-decanoyl cyclopentylamide (C10-CPA) was found to be the strongest quorum-sensing inhibitor that interferes with expression of Pseudomonas aeruginosa virulence factors regulated by the las and rhl quorum-sensing systems.

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Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp strain A28

Article Abstract:

Research reveal that extracellular product of the marine bacterium Pseudoalteromonas sp strain A28 possesses algicidal activity mediated by protease. Studies with the purified enzyme and protease minus mutants show that extracellularly produced serine protease is responsible for the algicidal activity against the diatom.

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