Development of PCR assays targeting genes in O-Antigen gene clusters for detection and identification of Escherichia coli O45 and O55 serogroups

Article Abstract:

The rapid and specific PCR assays for detecting and typing Escherichia coli serogroups O45 and O55 by targeting the wzx and wzy genes of the respective O-antigen gene clusters was developed. It shows that the PCR assays were used to detect E. coli O45 and O55 serogroup strains in spiked fecal samples and can potentially be used for detecting the presence of these serogroups in food, fecal and environmental samples.

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Detection of Escherichia coli serogroups O26 and O113 by PCR amplification of the wzx and wzy genes

Article Abstract:

A detailed examination of PCR-based assays for detecting enterohemorrhagic Escherichia coli serogroups O26 and O113 that were developed by targeting the wzx and wzy genes found in the O-antigen gene cluster of each organism, is made. The PCR assays were specific for the respective serogroups, as there was no amplification of DNA from non-O26 and non-O113 E. coli serogroups or from other bacterial genera tested.

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Prevalence and characterization of Shiga toxin-producing escherichia coli in swine feces recovered in the national animal health monitoring system's swine 2000 study

Article Abstract:

A study is conducted to determine the prevalence of Shiga toxin-producing Escherichia coli (STEC) in swine feces as a part of the National Animal Health Monitoring System's Swine 2000 study. The findings indicate that swine in the United States harbor STEC that can potentially cause human illness.

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