Development and characterization of a subtilin-regulated expression system in Bacillus subtilis: Strict control of gene expression by addition of subtilin

Article Abstract:

The construction of a subtilin-regulated gene expression (SURE) system for Bacillus subtilis in which spaR- and spaK-dependent signal transduction is used to control P(sub spac)-driven gene expression is described. The results show that the SURE system provides a strictly controlled and effective gene expression toolbox for Bacillus subtilis and it can also provide a suitable expression system for various scientific and industrial applications.

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Controlled gene expression systems for lactic acid bacteria: transferable nisin-inducible expression cassettes for Lactococcus, Leuconostoc, and Lactobacillus spp

Article Abstract:

A dual-plasmid inducible gene expression system was elaborated for lactic acid bacteria. This transferable gene expression system is based on the autoregulatory expression of nisin, a microbial peptide widely used as a natural preservative in the food industry. Nisin-inducible gene expression was carried out in Lactococcus lactis, Leuconostoc lactis and Lactobacillus helveticus with the introduction of the dual-plasmid system.

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Production and secretion stress caused by overexpression of heterologous alpha-amylase leads to inhibition of sporulation and a prolonged motile phase in bacillus subtilis

Article Abstract:

Transcriptome analysis is used to investigate the global stress response of the gram-positive bacterium Bacillus subtilis. The results predicts that initiation of sporulation and mobility play an important role in combating protein secretion stress.

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