Determination of plasmid DNA concentration maintained by nonculturable Escherichia coli in marine microcosms

Article Abstract:

The plasmid pBR322 DNA concentration maintained by nonculturable Escherichia coli JM83 in marine microcosms was determined. The cells maintained the plasmid for 28 days in artificial seawater and reached nonculturability within 7 days. Although cells inoculated into natural seawater and estuarine water showed different culturability, the maintenance of the plasmid was not affected. Thus, vector plasmids in low copy number per cell could be maintained in nonculturable E. coli cells.

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Optimization of analytical parameters for inferring relationships among Escherichia coli isolates from repetitive-element PCR by maximizing correspondence with multilocus sequence typing data

Article Abstract:

A multilocus sequence typing (MLST) is used as a 'gold standard' to optimize the analytical parameters for inferring relationships among Escherichia coli isolates from repetitive-element PCR (rep-PCR) data. The results indicate that rep-PCR yields accurate information for inferring relationships among E. coli isolates and that accuracy can be enhanced with the use of analytical methods that consider the shapes of densitometric profiles.

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Direct extraction of bacterial plasmids from food for polymerase chain reaction amplification

Article Abstract:

The elimination of polymerase chain reaction-inhibiting components present in foods and enrichment media was evaluated by several plasmid purification schemes. The procedure was applied to14 foods that were contaminated with an overnight culture of Enteroinvasive Escherichia coli strain. The technique was able to efficiently remove polymerase inhibitors present in both foods and enrichment media.

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