Determination of active marine bacterioplankton: a comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining

Article Abstract:

Several techniques for the assessment of bacterial numbers and activity in marine waters were compared utilizing samples from a number of marine environments, from aged offshore seawater to rich harbor water. Samples were simultaneously tested for binding to a fluorescently labeled universal 16S rRNA probe; 3H-labeled amino acid uptake via autoradiography; nucleoid-containing bacterial numbers by modified 4',6-diamidino-2-phenylindole staining; staining with 5-cyano-2,3-ditolyl tetrazolium chloride; and total bacterial counts taken as reference.

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Prevalence of the chloroflexi-related SAR202 bacterioplakton cluster throughout the mesopelagic zone and Deep Ocean

Article Abstract:

Newly identified SAR202-related 16S rRNA gene sequences are used to further resolve the phylogeny of the cluster and to design taxon-specific oligonucleotide probes for fluorescence in situ hybridization. On average, members of the SAR202 group accounted for 10.2 percent of all DNA-containing bacterioplankton between 500 and 4,000m.

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Richness and diversity of bacterioplankton species along an estuarine gradient in Moreton Bay, Australia

Article Abstract:

Moreton Bay, Australia, consists of vast bacterioplankton community. This community is examined using rRNA intergenic spacer analysis, the results of which are discussed.

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