Detection of viable Giardia cysts by amplification of heat shock-induced mRNA

Article Abstract:

PCR amplifications of heat shock-induced mRNA sequences that utilize the heat shock proteins, can effectively detect viable Giardia cysts in surface water samples. The PCR amplification of DNA or the reverse transcription-PCR (RT-PCR) amplification of the corresponding RNA sequences yields a 163-bp product. The amplifications indicate only a presence/absence test. However, viable Giardia cysts can be detected with amplification of heat-shock-induced mRNA with the same primers. The differences between PCR and RT-PCR, and the sensitivity and viability of the amplifications are discussed.

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Confirmation of the human-pathogenic microsporidia Enteocytozoon bieneusi, Encephalitozoon intestinalis, and Vittaforma corneae in water

Article Abstract:

The confirmation of the human-pathogenic microsporidia Enteocytozoon bieneusi, Encephalitozoon intestinalis, and Vittaforma corneae in water was conducted. The study was undertaken with the objective of screening various significant water sources. Community DNA extraction was followed by microsporidium-specific PCR amplification, PCR sequencing and database homology comparison. Results suggest that the human-pathogenic microsporidia may be waterborne pathogens.

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Persistence of viruses in desert soils amended with anaerobically digested sewage sludge

Article Abstract:

Land application of sewage sludge was studiedin Pima County, Arizona. Factors observed that influenced survival of sludge viruses were soil type, moisture and temperature. Clay soils decreased survivalof viruses than sandy soils while moist soil conditions and low temperatures enhanced their activation.

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