Detection of toxin-producing cyanobacteria by use of paramagnetic beads for cell concentration and DNA purification

Article Abstract:

A study was conducted to create an integrated cell concentration and DNA purification assay for early detection of potential toxic water blooms formed by cyanobacteria in environmental water. A simple nucleic-acid based method was devised to facilitate detection of cyanobacteria in water using the same paramagnetic solid phase for both bacterial cell concentration and subsequent DNA purification. This technique is recommended for routing environmental monitoring.

author: Jakobsen, Kjetill S., Rudi, Knut, Larsen, Frank
Research, Methods, Cyanobacteria, Environmental monitoring, Microbiological assay

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Direct real-time PCR quantification of Campylobacter jejuni in chicken fecal and cecal samples by integrated cell concentration and DNA purification

Article Abstract:

One of the main causes of diarrheal disease or food-based gastroenteritis is campylobacter jejuni, which is a microorganism isolated from poultry, cattle and pigs among other animals. Tests were conducted to understand the colonization kinetics in poultry, which could help in controlling human infections by these bacteria.

author: Jakobsen, Kjetill S., Rudi, Knut, Hoidal, Hilde Kristin, Katla, Tone, Johansen, Birgit Klungseth, Nordal, John
Product information, Testing, Risk factors, Diarrhea, Poultry, Poultry (Animals)

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Explorative multifactor approach for investigating global survival mechanisms of Campylobacter jejuni under environmental conditions

Article Abstract:

An investigation of the global survival mechanism of Campylobacter jejuni in the environment is conducted using the novel multivariate analysis of variance (MANOVA) approach. The results suggest that the amount of polysaccharides and oligosaccharides increases under the nongrowth survival conditions.

author: Rudi, Knut, Moen, Birgitte, Oust, Astrid, Langsrud, Oyvind, Dorrell, Nick, Kohler, Achim, Wren, Bredan W., Marsden, Gemma L., Hinds, Jason
All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Polysaccharides, Analysis, Environmental aspects, Multivariate analysis, Oligosaccharides

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subjects list: Campylobacter
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