Article Abstract:
The methanotrophs have a lot of biotechnological potential and are important in the global methane cycle. In an experiment, methane-oxidizing bacteria were utilized to assess the specificity and efficiency of rRNA-targeted group-specific probes. Methanotroph group specific probes did not give a signal with samples from these enrichments, though a methanol dehydrogenase structural gene was detected. Enrichment on methane combined with the screening with the broad specific methanol dehydrogenase probe facilitated detection of novel methanotrophs that were not detected with the original suit of methanotroph group-specific probes.
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Article Abstract:
A study shows that the chitosanase gene, encoding the enzyme chitosanase from actinomycete Nocardioides species N106, is a third member of glycosyl hydrolase family 46. The enzyme has a specific activity of 63 U per mg, a molecular mass of 29.5 kDa and participates in chitosan degradation. A combination of two enzymes, with a difference of one N-terminal amino acid, forms the mature chitosanase. The chitosanase gene from N106 and Streptomyces species N174 are identical by 79.5%.
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Article Abstract:
The inlA gene encodes for the 80 kDa protein internalin in the gram-positive bacterium Listeria monocytogenes. Internalin allows the bacterium to multiply in host cells. The distribution and the DNA polymorphisms of inlA sequences in various wild strains of L. monocytogenes are compared to other Listeria species. Results indicate that L. monocytogenes is the only Listeria specie in which inlA required for cell invasion is expressed.
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