Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction, and hybridization

Article Abstract:

The utilization of reverse transcription and polymerase chain reaction for the detection in activated sludge and surface waters of enteroviruses is discussed. RT-PCR was compared against direct hybridization and cell culture methods. The sensitivity of RT-PCR was overwhelmingly better compared to the other two. However, contaminants in activated sludge and concentrated surface waters must be monitored to avoid the inhibition of its enzymatic amplification.

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Analysis of the gene family encoding lipases in Candida rugosa by competitive reverse transcription-PCR

Article Abstract:

Research was conducted to illustrate the synthesis of multiple extracellular lipases in Candida rugosa. To quantify the expression of lip genes, a competitive reverse transcription-PCR assay was developed. Using the approach made it possible to demonstrate the differential expression of the five lip genes in the presence of different inducers that are known to be able to increase C rugosa lipase production.

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Detection of acute bee paralysis virus and black queen cell virus from honeybees by reverse transcriptase PCR

Article Abstract:

Reverse transcriptase PCR was used to identify acute bee paralysis virus (ABPV) and black queen cell virus (BQCV) with sensitivities of 1,600 genome equivalents of ABPV and 130 genome equivalents of BQCV. This method is more rapid than serological methods.

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