Detection of active yeast cells (Saccharomyces cerevisiae) in frozen dough sections

Article Abstract:

A new method for counting active yeast cells in frozen dough sections was described. Double staining with 4',6-diamidino-2-phenylindole and Evans blue and fluorescence detection allowed differentiation between dead and living yeast cells. The counting of active yeast cells after fermentation correlated well with the gas-producing capability of the dough. However, correlation between this method and the conventional method of plating of the yeast cells was poor.

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Intrinsic properties of so-called dormant probiotic bacteria, determined by flow cytometric viability assays

Article Abstract:

Plate counting and four culture-independent flow cytometric assays were used for determining the viability and intrinsic properties of three so-called probiotic strains while storage. The strains demonstrated reduction in plate counts but could maintain esterase activity, intact cytoplasmic membrane and pH gradient.

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Adhesion of bifidobacteria to granular starch and its implications in probiotic technologies

Article Abstract:

Many bifidobacteria can adhere to starch granules, which could be used to microencapsulate the bacteria for use as probiotics. The adhesion appears to involve alpha-1,4-linked glucose sugars and is reduced by protease, pancreatin, and pH values of 3 or less.

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