Detection and identification by PCR of a highly virulent phylogenetic subgroup among extraintestinal pathogenic Escherichia coli B2 strains

Article Abstract:

A DNA pathoarray analysis is used for developing a PCR specific for Escherichia coli B2 strains (O1:K1, O2:K1, O18:K1 and O45:K1) subgroup, which is included in the multiplex phylogenetic-grouping PCR method. This new PCR method can be used for identifying this virulent subgroup among different ecological niches.

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Improved succinic acid production in the anaerobic culture of an Escherichia coli pflB ldhA double mutant as a result of enhanced anaplerotic activities in the preceding aerobic culture

Article Abstract:

Two-stage culture of Escherichia coli NZN111 is carried out for the production of succinic acid. The results have shown that the high efficiency of succinic acid production has resulted from increased anaplerotic activities that have made regeneration of [NAD.sup.+] from NADH possible.

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Use of the tna operon as a new molecular target for Escherichia coli detection

Article Abstract:

A quantitative real time PCR was developed to detect pathogenic and nonpathogenic strains of Escherichia coli differentiating them from Shigella spp. Results concluded that Shigella pathotype originated from Escherichia coli ancestors.

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